Digital Histology Facility

The TIGA's Digital Histology Facility provides access to the following services:

Wet lab services

• Tissue processing
• Tissue sectioning (Cryo and FFPE samples)
• Tissue staining (brightfield, fluorescence)

Scanning services

• Scanning of brightfield or fluorescent samples
• Digitization in 20x or 40x magnification

Image analysis services

Depending on the complexity of the projects, we use software frameworks to perform the following image analysis tasks:

TMA (Tissue-microarray) quantification:

• TMAs stained in brightfield
• TMAs stained in fluorescence
  
  

Complete Cell Quantification

Quantification of expression levels of:

• Nuclei
• Membranous stains
• Cytoplasm
  
  

Biomarker-scoring analysis examples:

• Her2 Scoring
• ER, PR
• Development of new scoring systems
  
  

Examples for spatial analysis in immune cell profiling

• Detection of tumor infiltrating leukocytes (TILs) and quantification of expression levels of different markers
• Measurement of different TIL-areas.
• Segmentation of tissue areas improves the analysis with additional spatial data (how far are the infiltrating clusters away from the tumor/stroma)
  
  

Examples for complex object analysis:

• Vessels
• Microvessels
• Rare Event detection
• Morphological measurements

How to contact

Scientists interested in using the facility should contact us by e-mail:

niels.grabe[at]bioquant.uni-heidelberg.de

We will contact you as soon as poosible to make an appointment for discussing the project and the affilated requirements.